Certificate of Analysis for Chicken Bone Powder

Certificate of Analysis for Chicken Bone Powder

Product Information

1. Physical and Chemical Analysis

This table outlines the nutritional and physical properties of the chicken bone powder, ensuring it meets quality standards for composition and appearance.

Table 1: Physical and Chemical Analysis

Parameter	Specification	Result	Test Method	Compliance
Appearance	Fine white to light gray powder	Fine white powder	Visual Inspection	Pass
Odor	Neutral, no off-odors	Neutral	Sensory Evaluation	Pass
Protein (%)	20.0–30.0	25.2	Kjeldahl Method (AOAC 981.10)	Pass
Fat (%)	≤8.0	4.8	Soxhlet Extraction (AOAC 920.39)	Pass
Ash (%)	≥50.0	55.5	Gravimetric Method (AOAC 942.05)	Pass
Moisture (%)	≤5.0	3.9	Oven Drying (AOAC 930.15)	Pass
Calcium (%)	≥20.0	22.3	ICP-MS (AOAC 2011.14)	Pass
Phosphorus (%)	≥10.0	11.2	ICP-MS (AOAC 2011.14)	Pass
Ca:P Ratio	1.5:1 to 2:1	1.99:1	Calculated	Pass

Analysis: The chicken bone powder meets specifications for physical and chemical properties. Its high ash (55.5%) and calcium (22.3%) content confirm its suitability as a mineral source, while low moisture (3.9%) ensures shelf stability.

2. Microbiological Analysis

This table ensures the product is safe for use by verifying the absence of harmful pathogens and low microbial counts.

Table 2: Microbiological Analysis

Parameter	Specification	Result	Test Method	Compliance
Total Plate Count (CFU/g)	≤10,000	4,500	Plate Count (AOAC 990.12)	Pass
Coliforms (CFU/g)	≤10	<10	MPN Method (AOAC 991.14)	Pass
Salmonella (in 25g)	Absent	Absent	Enrichment (AOAC 2004.03)	Pass
E. coli (CFU/g)	≤10	<10	MPN Method (AOAC 991.14)	Pass
Yeast and Mold (CFU/g)	≤100	30	Plate Count (AOAC 997.02)	Pass

Analysis: The microbiological results confirm the product’s safety, with low microbial counts and absence of pathogens, meeting AAFCO and GB 29921 standards.

3. Heavy Metal and Contaminant Analysis

This table checks for heavy metals and other contaminants, as bones can accumulate toxins during the animal’s life.

Table 3: Heavy Metal and Contaminant Analysis

Parameter	Specification	Result	Test Method	Compliance
Lead (mg/kg)	≤5.0	2.4	ICP-MS (AOAC 2013.06)	Pass
Cadmium (mg/kg)	≤1.0	0.3	ICP-MS (AOAC 2013.06)	Pass
Mercury (mg/kg)	≤0.1	0.02	ICP-MS (AOAC 2013.06)	Pass
Arsenic (mg/kg)	≤2.0	0.5	ICP-MS (AOAC 2013.06)	Pass
Antibiotic Residues (e.g., Tetracycline, mg/kg)	≤0.1	Not Detected	HPLC (AOAC 995.09)	Pass

Analysis: Heavy metal levels are well below the maximum limits, indicating the product is safe for use. The low lead level (2.4 mg/kg) aligns with the prior analysis of chicken bone powder’s relatively low bioaccumulation risk compared to organ-based ingredients.

4. Additional Quality Parameters

This table includes additional quality checks, such as particle size and absence of additives, to ensure the product meets “pure natural” claims.

Table 4: Additional Quality Parameters

Parameter	Specification	Result	Test Method	Compliance
Particle Size (Mesh)	95% pass through 80 mesh	98%	Sieve Analysis (ASTM D1921)	Pass
Color	White to light gray	Light gray	Visual Inspection	Pass
Artificial Additives	Absent	Absent	HPLC/GC-MS (AOAC 2014.10)	Pass
Digestibility (%)	60–70	66	In Vitro Digestibility (AAFCO Protocol)	Pass

Analysis: The powder’s fine particle size (98% passing 80 mesh) ensures uniformity in application, and the absence of additives confirms its “pure natural” status. Digestibility (66%) aligns with the expected range for a mineral-focused ingredient.

5. Comparison with Previous Ingredients

To provide context, this table compares key CoA parameters of chicken bone powder with chicken liver powder, chicken meal, and poultry by-product meal, using data from prior analyses.

Table 5: CoA Parameter Comparison

Parameter	Chicken Bone Powder	Chicken Liver Powder	Chicken Meal	Poultry By-Product Meal
Protein (%)	25.2	65.0	68.0	62.0
Ash (%)	55.5	8.0	11.0	14.0
Calcium (%)	22.3	0.5	3.5	4.5
Total Plate Count (CFU/g)	4,500	5,000	4,000	6,000
Lead (mg/kg)	2.4	3.0	2.0	4.0
Digestibility (%)	66	80	83	72

Analysis: Chicken bone powder stands out for its high ash (55.5%) and calcium (22.3%), but it has the lowest protein (25.2%) and digestibility (66%). Its lead level (2.4 mg/kg) is lower than chicken liver powder (3.0 mg/kg) and poultry by-product meal (4.0 mg/kg), reflecting its safer profile for heavy metals.

6. Conclusion

The Certificate of Analysis for this batch of chicken bone powder confirms that it meets all specified standards for physical, chemical, microbiological, and safety parameters. Key highlights include:

This CoA provides a comprehensive, data-driven assurance of quality, aligning with industry standards and the user’s request for detailed tables and scientific analysis. If further customization or additional parameters are needed, please let me know!